› 4uot

X-ray diffraction
1.69Å resolution

Thermodynamic hyperstability in parametrically designed helical bundles

Released:
DOI: 10.2210/pdb4uot/pdb
PDB entry 4uot coloured by chain, front view.
PDB entry 4uot coloured by chain, side view.
PDB entry 4uot coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
High thermodynamic stability of parametrically designed helical bundles.
Huang PS, Oberdorfer G, Xu C, Pei XY, Nannenga BL, Rogers JM, DiMaio F, Gonen T, Luisi B, Baker D
Science 346 481-485 (2014)
PMID: 25342806

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165232 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DESIGNED HELICAL BUNDLE 5H2L Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 34 amino acids
Theoretical weight: 4.14 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2221
Unit cell:
a: 55.4Å b: 88.02Å c: 103.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 0.184
Expression system: Not provided

Quick links

Citations

35 review citations

The coming of age of de novo protein design.
Huang et al. (2016)
34 more

1 mention without citation

High thermodynamic stability of parametrically designed helical bundles.
Huang et al. (2014)