› 4wcw

X-ray diffraction
2.1Å resolution

Ribosomal silencing factor during starvation or stationary phase (RsfS) from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb4wcw/pdb
PDB entry 4wcw coloured by chain, front view.
PDB entry 4wcw coloured by chain, side view.
PDB entry 4wcw coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structure of Ribosomal Silencing Factor Bound to Mycobacterium tuberculosis Ribosome.
Li X, Sun Q, Jiang C, Yang K, Hung LW, Zhang J, Sacchettini JC
Structure 23 1858-1865 (2015)
PMID: 26299947

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131469 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal silencing factor RsfS Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 139 amino acids
Theoretical weight: 15.28 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Mycolicibacterium smegmatis
UniProt:
  • Canonical: O86327 (Residues: 1-126; Coverage: 100%)
Gene names: Rv2420c, rsfS
Sequence domains: Ribosomal silencing factor during starvation
Structure domains: Beta Polymerase, domain 2

Ligands and Environments

2 bound ligands:
Ligand MG 6 x MG
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P1
Unit cell:
a: 50.975Å b: 50.975Å c: 64.546Å
α: 110.27° β: 96.17° γ: 110.59°
R-values:
R R work R free
0.213 0.21 0.256
Expression system: Mycolicibacterium smegmatis

Quick links

Citations

4 review citations

Ribosome Hibernation.
Prossliner et al. (2018)
3 more

4 mentions without citation

Production of recombinant proteins in Mycobacterium smegmatis for structural and functional studies.
Bashiri et al. (2015)
3 more