› 4wok

X-ray diffraction
1.85Å resolution

Crystal structure of UDP-glucose 4-epimerase from Brucella ovis in complex with NAD

Released:
DOI: 10.2210/pdb4wok/pdb
PDB entry 4wok coloured by chain, front view.
PDB entry 4wok coloured by chain, side view.
PDB entry 4wok coloured by chain, top view.
Source organism: Brucella ovis ATCC 25840
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose = UDP-alpha-D-galactose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101793 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucose 4-epimerase Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 36.26 KDa
Source organism: Brucella ovis ATCC 25840
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3ASR6 (Residues: 1-328; Coverage: 100%)
Gene names: BOV_A0474, galE-1
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
2 bound ligands:
Ligand ZN 1 x ZN
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: I222
Unit cell:
a: 76.48Å b: 86.46Å c: 126.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.167 0.199
Expression system: Escherichia coli BL21(DE3)

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