› 4x65

X-ray diffraction
3.34Å resolution

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Released:
DOI: 10.2210/pdb4x65/pdb
PDB entry 4x65 coloured by chain, front view.
PDB entry 4x65 coloured by chain, side view.
PDB entry 4x65 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
N(6)-methyladenosine in mRNA disrupts tRNA selection and translation-elongation dynamics.
Choi J, Ieong KW, Demirci H, Chen J, Petrov A, Prabhakar A, O'Leary SE, Dominissini D, Rechavi G, Soltis SM, Ehrenberg M, Puglisi JD
OpenAccess logo Nat Struct Mol Biol 23 110-5 (2016)
PMID: 26751643

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 23-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-250130 (preferred)
Entry contents:
20 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (23 distinct):
Small ribosomal subunit protein uS2 Chain: B
Molecule details ›
Chain: B
Length: 236 amino acids
Theoretical weight: 27.22 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80371 (Residues: 6-241; Coverage: 92%)
Gene names: TTHA0861, rps2, rpsB
Sequence domains: Ribosomal protein S2
Structure domains:
Small ribosomal subunit protein uS3 Chain: C
Molecule details ›
Chain: C
Length: 207 amino acids
Theoretical weight: 22.98 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80372 (Residues: 2-208; Coverage: 87%)
Gene names: TTHA1686, rps3, rpsC
Sequence domains:
Structure domains:
Small ribosomal subunit protein uS4 Chain: D
Molecule details ›
Chain: D
Length: 208 amino acids
Theoretical weight: 24.24 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80373 (Residues: 2-209; Coverage: 100%)
Gene names: TTHA1665, rps4, rpsD
Sequence domains:
Structure domains:
Small ribosomal subunit protein uS5 Chain: E
Molecule details ›
Chain: E
Length: 151 amino acids
Theoretical weight: 16.46 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHQ5 (Residues: 5-155; Coverage: 93%)
Gene names: TTHA1675, rpsE
Sequence domains:
Structure domains:
Small ribosomal subunit protein bS6 Chain: F
Molecule details ›
Chain: F
Length: 101 amino acids
Theoretical weight: 11.99 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SLP8 (Residues: 1-101; Coverage: 100%)
Gene names: TTHA0245, rpsF
Sequence domains: Ribosomal protein S6
Structure domains: Alpha-Beta Plaits
Small ribosomal subunit protein uS7 Chain: G
Molecule details ›
Chain: G
Length: 155 amino acids
Theoretical weight: 17.92 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P17291 (Residues: 2-156; Coverage: 99%)
Gene names: TTHA1696, rps7, rpsG
Sequence domains: Ribosomal protein S7p/S5e
Structure domains: Ribosomal protein S7 domain
Small ribosomal subunit protein uS8 Chain: H
Molecule details ›
Chain: H
Length: 138 amino acids
Theoretical weight: 15.87 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P0DOY9 (Residues: 1-138; Coverage: 100%)
Gene names: TTHA1678, rpsH
Sequence domains: Ribosomal protein S8
Structure domains:
Small ribosomal subunit protein uS9 Chain: I
Molecule details ›
Chain: I
Length: 127 amino acids
Theoretical weight: 14.28 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80374 (Residues: 2-128; Coverage: 99%)
Gene names: TTHA1464, rps9, rpsI
Sequence domains: Ribosomal protein S9/S16
Structure domains: Ribosomal Protein S5; domain 2
Small ribosomal subunit protein uS10 Chain: J
Molecule details ›
Chain: J
Length: 99 amino acids
Theoretical weight: 11.4 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHN7 (Residues: 3-101; Coverage: 94%)
Gene names: TTHA1693, rpsJ
Sequence domains: Ribosomal protein S10p/S20e
Structure domains: Ribosomal protein S10 domain
Small ribosomal subunit protein uS11 Chain: K
Molecule details ›
Chain: K
Length: 119 amino acids
Theoretical weight: 12.61 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80376 (Residues: 11-129; Coverage: 92%)
Gene names: TTHA1666, rps11, rpsK
Sequence domains: Ribosomal protein S11
Structure domains: Ribosomal protein S11
Small ribosomal subunit protein uS12 Chain: L
Molecule details ›
Chain: L
Length: 125 amino acids
Theoretical weight: 13.92 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHN3 (Residues: 2-126; Coverage: 95%)
Gene names: TTHA1697, rpsL
Sequence domains: Ribosomal protein S12/S23
Structure domains: Nucleic acid-binding proteins
Small ribosomal subunit protein uS13 Chain: M
Molecule details ›
Chain: M
Length: 118 amino acids
Theoretical weight: 13.37 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80377 (Residues: 2-119; Coverage: 94%)
Gene names: TTHA1667, rps13, rpsM
Sequence domains: Ribosomal protein S13/S18
Structure domains:
Small ribosomal subunit protein uS14 Chain: N
Molecule details ›
Chain: N
Length: 60 amino acids
Theoretical weight: 7.03 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P0DOY6 (Residues: 2-61; Coverage: 98%)
Gene names: TTHA1679, rpsN, rpsZ
Sequence domains: Ribosomal protein S14p/S29e
Structure domains: 30s Ribosomal Protein S14; Chain N
Small ribosomal subunit protein uS15 Chain: O
Molecule details ›
Chain: O
Length: 88 amino acids
Theoretical weight: 10.45 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJ76 (Residues: 2-89; Coverage: 99%)
Gene names: TTHA1138, rpsO
Sequence domains: Ribosomal protein S15
Structure domains: S15/NS1, RNA-binding
Small ribosomal subunit protein bS16 Chain: P
Molecule details ›
Chain: P
Length: 84 amino acids
Theoretical weight: 10 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJH3 (Residues: 1-84; Coverage: 96%)
Gene names: TTHA1035, rpsP
Sequence domains: Ribosomal protein S16
Structure domains: S16 Ribosomal Protein; Chain: A;
Small ribosomal subunit protein uS17 Chain: Q
Molecule details ›
Chain: Q
Length: 99 amino acids
Theoretical weight: 11.72 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P0DOY7 (Residues: 2-100; Coverage: 94%)
Gene names: TTHA1683, rpsQ
Sequence domains: Ribosomal protein S17
Structure domains: Nucleic acid-binding proteins
Small ribosomal subunit protein bS18 Chain: R
Molecule details ›
Chain: R
Length: 73 amino acids
Theoretical weight: 8.5 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SLQ0 (Residues: 16-88; Coverage: 83%)
Gene names: TTHA0243, rpsR
Sequence domains: Ribosomal protein S18
Structure domains: Ribosomal protein S18
Small ribosomal subunit protein uS19 Chain: S
Molecule details ›
Chain: S
Length: 81 amino acids
Theoretical weight: 9.26 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHP2 (Residues: 2-82; Coverage: 87%)
Gene names: TTHA1688, rpsS
Sequence domains: Ribosomal protein S19
Structure domains: 30s Ribosomal Protein S19; Chain A
Small ribosomal subunit protein bS20 Chain: T
Molecule details ›
Chain: T
Length: 99 amino acids
Theoretical weight: 10.92 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80380 (Residues: 8-106; Coverage: 93%)
Gene names: TTHA1397, rps20, rpsT
Sequence domains: Ribosomal protein S20
Structure domains: Ribosomal protein S20
Small ribosomal subunit protein bTHX Chain: U
Molecule details ›
Chain: U
Length: 25 amino acids
Theoretical weight: 3.09 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SIH3 (Residues: 2-26; Coverage: 93%)
Gene names: TTHA1396, rpsU
Sequence domains: 30S ribosomal protein Thx
16S ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 1522 nucleotides
Theoretical weight: 494.17 KDa
Sequence domains: Bacterial small subunit ribosomal RNA
RNA (5'-D(*AP*(MA6)P*AP*UP*UP*U)-3') Chain: a
Molecule details ›
Chain: a
Length: 6 nucleotides
Theoretical weight: 1.88 KDa
RNA (5'-D(P*GP*AP*CP*UP*(70U)P*UP*UP*(12A)P*AP*(PSU)P*C)-3') Chain: b
Molecule details ›
Chain: b
Length: 11 nucleotides
Theoretical weight: 3.71 KDa

Ligands and Environments

4 bound ligands:
Ligand PAR 6 x PAR
Ligand MG 317 x MG
Ligand K 46 x K
Ligand ZN 2 x ZN
12 modified residues:
Ligand PSU 4 x PSU
Ligand 7MG 1 x 7MG
Ligand M2G 1 x M2G
Ligand 5MC 4 x 5MC
Ligand 2MG 1 x 2MG
Ligand 4OC 1 x 4OC
Ligand UR3 1 x UR3
Ligand MA6 2 x MA6
Ligand 0TD 1 x 0TD
Ligand 6MZ 1 x 6MZ
Ligand 70U 1 x 70U
Ligand 12A 1 x 12A

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P41212
Unit cell:
a: 401.44Å b: 401.44Å c: 175.924Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.21

Quick links

Citations

68 review citations

Post-transcriptional gene regulation by mRNA modifications.
Zhao et al. (2017)
67 more

1 mention without citation

N(6)-methyladenosine in mRNA disrupts tRNA selection and translation-elongation dynamics.
Choi et al. (2016)