› 4x88

X-ray diffraction
3.5Å resolution

E178D Selectivity filter mutant of NavMS voltage-gated pore

Released:
DOI: 10.2210/pdb4x88/pdb
PDB entry 4x88 coloured by chain, front view.
PDB entry 4x88 coloured by chain, side view.
PDB entry 4x88 coloured by chain, top view.
Source organism: Magnetococcus marinus MC-1
Primary publication:
Molecular basis of ion permeability in a voltage-gated sodium channel.
Naylor CE, Bagnéris C, DeCaen PG, Sula A, Scaglione A, Clapham DE, Wallace BA
OpenAccess logo EMBO J 35 820-30 (2016)
PMID: 26873592

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106115 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ion transport domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 149 amino acids
Theoretical weight: 16.82 KDa
Source organism: Magnetococcus marinus MC-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0L5S6 (Residues: 130-274; Coverage: 53%)
Gene name: Mmc1_0798
Sequence domains:
Structure domains: Helix Hairpins

Ligands and Environments

3 bound ligands:
Ligand 2CV 4 x 2CV
Ligand 1PE 4 x 1PE
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2221
Unit cell:
a: 80.23Å b: 336.02Å c: 80.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.205
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

The chemical basis for electrical signaling.
Catterall et al. (2017)
14 more

2 mentions without citation

Molecular basis of ion permeability in a voltage-gated sodium channel.
Naylor et al. (2016)
1 more