› 4z3f

X-ray diffraction
1.8Å resolution

Crystal structure of the lectin domain of PapG from E. coli BI47 in complex with SSEA4 in space group P21

Released:
DOI: 10.2210/pdb4z3f/pdb
PDB entry 4z3f coloured by chain, front view.
PDB entry 4z3f coloured by chain, side view.
PDB entry 4z3f coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Carbohydrate-Lectin Interactions: An Unexpected Contribution to Affinity.
Navarra G, Zihlmann P, Jakob RP, Stangier K, Preston RC, Rabbani S, Smiesko M, Wagner B, Maier T, Ernst B
Chembiochem 18 539-544 (2017)
PMID: 28076665

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
PapG carbohydrate-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 198 amino acids
Theoretical weight: 22.76 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A182DW20 (Residues: 2-198; Coverage: 100%)
Gene names: G801_04654, G801_04690
Sequence domains: PapG carbohydrate binding domain
Structure domains: Bacterial adhesin receptor binding domain

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL, GLA, NGA, SIA
Carbohydrate polymer
1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 45.11Å b: 53.38Å c: 48.82Å
α: 90° β: 96.05° γ: 90°
R-values:
R R work R free
0.202 0.2 0.225
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Conformational Studies of Oligosaccharides.
Yu et al. (2020)
2 more