› 5aom

X-ray diffraction
1.74Å resolution

Structure of the p53 cancer mutant Y220C with bound small molecule PhiKan883

Released:
DOI: 10.2210/pdb5aom/pdb
PDB entry 5aom coloured by chain, front view.
PDB entry 5aom coloured by chain, side view.
PDB entry 5aom coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Exploiting Transient Protein States for the Design of Small-Molecule Stabilizers of Mutant p53.
Joerger AC, Bauer MR, Wilcken R, Baud MGJ, Harbrecht H, Exner TE, Boeckler FM, Spencer J, Fersht AR
OpenAccess logo Structure 23 2246-2255 (2015)
PMID: 26636255

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138105 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellular tumor antigen p53 Chains: A, B
Molecule details ›
Chains: A, B
Length: 219 amino acids
Theoretical weight: 24.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04637 (Residues: 94-312; Coverage: 56%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like

Ligands and Environments

3 bound ligands:
Ligand FY8 4 x FY8
Ligand GOL 1 x GOL
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 65.16Å b: 71.27Å c: 105.11Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.191
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The p53 Pathway: Origins, Inactivation in Cancer, and Emerging Therapeutic Approaches.
Joerger et al. (2016)
7 more

2 mentions without citation

Exploiting Transient Protein States for the Design of Small-Molecule Stabilizers of Mutant p53.
Joerger et al. (2015)
1 more