› 5ihl

X-ray diffraction
3.3Å resolution

STRUCTURE OF THE EXTRACELLULAR DOMAIN OF THE CD40 IN COMPLEX WITH 3H56-5 DAB

Released:
DOI: 10.2210/pdb5ihl/pdb
PDB entry 5ihl coloured by chain, front view.
PDB entry 5ihl coloured by chain, side view.
PDB entry 5ihl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Functional Antagonism of Human CD40 Achieved by Targeting a Unique Species-Specific Epitope.
Yamniuk AP, Suri A, Krystek SR, Tamura J, Ramamurthy V, Kuhn R, Carroll K, Fleener C, Ryseck R, Cheng L, An Y, Drew P, Grant S, Suchard SJ, Nadler SG, Bryson JW, Sheriff S
J Mol Biol 428 2860-79 (2016)
PMID: 27216500

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229928 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tumor necrosis factor receptor superfamily member 5 Chains: A, D, F, H
Molecule details ›
Chains: A, D, F, H
Length: 183 amino acids
Theoretical weight: 20.08 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P25942 (Residues: 23-193; Coverage: 67%)
Gene names: CD40, TNFRSF5
Sequence domains: TNFR/NGFR cysteine-rich region
Structure domains: Tumor Necrosis Factor Receptor, subunit A, domain 2
3H56-5 domain antibody (dAb) Chains: B, E, G, I
Molecule details ›
Chains: B, E, G, I
Length: 121 amino acids
Theoretical weight: 13.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: I222
Unit cell:
a: 156.6Å b: 158.3Å c: 200.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.269 0.268 0.298
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

4 review citations

OX40: Structure and function - What questions remain?
Willoughby et al. (2017)
3 more