› 5m2j

X-ray diffraction
1.9Å resolution

Complex between human TNF alpha and Llama VHH2

Released:
DOI: 10.2210/pdb5m2j/pdb
Model geometry
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PDB entry 5m2j coloured by chain, front view.
PDB entry 5m2j coloured by chain, side view.
PDB entry 5m2j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Bivalent Llama Single-Domain Antibody Fragments against Tumor Necrosis Factor Have Picomolar Potencies due to Intramolecular Interactions.
Beirnaert E, Desmyter A, Spinelli S, Lauwereys M, Aarden L, Dreier T, Loris R, Silence K, Pollet C, Cambillau C, de Haard H
OpenAccess logo Front Immunol 8 867 (2017)
PMID: 28824615

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-134285 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tumor necrosis factor, soluble form Chain: A
Molecule details ›
Chain: A
Length: 157 amino acids
Theoretical weight: 17.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01375 (Residues: 77-233; Coverage: 67%)
Gene names: TNF, TNFA, TNFSF2
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls
Anti-(ED-B) scFV Chain: D
Molecule details ›
Chain: D
Length: 115 amino acids
Theoretical weight: 12.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P63
Unit cell:
a: 87.31Å b: 87.31Å c: 62.73Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.16 0.158 0.196
Expression system: Escherichia coli BL21

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Citations

11 review citations

A New Venue of TNF Targeting.
Steeland et al. (2018)
10 more

18 mentions without citation

Therapeutic implications of quercetin and its derived-products in COVID-19 protection and prophylactic.
Ho et al. (2024)
17 more

PDB-REDO

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