› 5xuk

X-ray diffraction
2.3Å resolution

Crystal structure of Helicobacter pylori holo-[acyl-carrier-protein] synthase (AcpS) in complex with coenzyme A

Released:
DOI: 10.2210/pdb5xuk/pdb
PDB entry 5xuk coloured by chain, front view.
PDB entry 5xuk coloured by chain, side view.
PDB entry 5xuk coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Entry authors: Liao YP, Wang DL, Yin DP, Zhang QY, Wang YM, Wang DQ, Zhu HX, Chen S

Function and Biology Details

Reaction catalysed: 
CoA-(4'-phosphopantetheine) + an apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + an [acyl-carrier-protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127756 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Holo-[acyl-carrier-protein] synthase Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 13.02 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli
UniProt:
  • Canonical: O25488 (Residues: 1-119; Coverage: 100%)
Gene names: HP_0808, acpS
Sequence domains: 4'-phosphopantetheinyl transferase superfamily
Structure domains: 4'-phosphopantetheinyl transferase domain

Ligands and Environments

1 bound ligand:
Ligand COA 1 x COA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P321
Unit cell:
a: 93.34Å b: 93.34Å c: 43.02Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.228 0.277
Expression system: Escherichia coli

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