› 5a43

X-ray diffraction
2.58Å resolution

Crystal structure of a dual topology fluoride ion channel.

Released:
DOI: 10.2210/pdb5a43/pdb
PDB entry 5a43 coloured by chain, front view.
PDB entry 5a43 coloured by chain, side view.
PDB entry 5a43 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structures of a double-barrelled fluoride ion channel.
Stockbridge RB, Kolmakova-Partensky L, Shane T, Koide A, Koide S, Miller C, Newstead S
OpenAccess logo Nature 525 548-51 (2015)
PMID: 26344196

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110524 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fluoride-specific ion channel FluC Chains: A, B
Molecule details ›
Chains: A, B
Length: 126 amino acids
Theoretical weight: 13.86 KDa
Source organism: Escherichia coli S88
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B7LI20 (Residues: 1-126; Coverage: 100%)
Gene names: ECS88_p0015, crcB, fluC
Sequence domains: CrcB-like protein, Camphor Resistance (CrcB)
MONOBODIES Chains: C, D
Molecule details ›
Chains: C, D
Length: 96 amino acids
Theoretical weight: 10.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand F 2 x F
Ligand DMU 3 x DMU
Ligand NA 1 x NA
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P41
Unit cell:
a: 87.42Å b: 87.42Å c: 146.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Nanobodies as Probes for Protein Dynamics in Vitro and in Cells.
Dmitriev et al. (2016)
10 more

11 mentions without citation

A topologically diverse family of fluoride channels.
Macdonald et al. (2017)
10 more