› 5abz

X-ray diffraction
2.4Å resolution

Complex of the FimH lectin with a C-linked naphtyl alpha-D-mannoside in soaked trigonal crystals at 2.40 A resolution

Released:
DOI: 10.2210/pdb5abz/pdb
PDB entry 5abz coloured by chain, front view.
PDB entry 5abz coloured by chain, side view.
PDB entry 5abz coloured by chain, top view.
Source organism: Escherichia coli UTI89
Primary publication:
Structures of C-mannosylated anti-adhesives bound to the type 1 fimbrial FimH adhesin.
de Ruyck J, Lensink MF, Bouckaert J
OpenAccess logo IUCrJ 3 163-7 (2016)
PMID: 27158502

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140004 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type 1 fimbrin D-mannose specific adhesin Chains: A, B
Molecule details ›
Chains: A, B
Length: 158 amino acids
Theoretical weight: 16.92 KDa
Source organism: Escherichia coli UTI89
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08191 (Residues: 22-179; Coverage: 57%)
Gene names: JW4283, b4320, fimH
Sequence domains: FimH, mannose binding
Structure domains: Fimbrial-type adhesion domain

Ligands and Environments

2 bound ligands:
Ligand Z47 2 x Z47
Ligand NI 2 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P3121
Unit cell:
a: 90.83Å b: 90.83Å c: 79.87Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.139 0.135 0.206
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The Interaction of the Gut Microbiota with the Mucus Barrier in Health and Disease in Human.
Corfield AP. (2018)
2 more

2 mentions without citation

Sites for Dynamic Protein-Carbohydrate Interactions of O- and C-Linked Mannosides on the E. coli FimH Adhesin.
Touaibia et al. (2017)
1 more