› 5af7

X-ray diffraction
1.89Å resolution

3-Sulfinopropionyl-coenzyme A (3SP-CoA) desulfinase from Advenella mimigardefordensis DPN7T: crystal structure and function of a desulfinase with an acyl-CoA dehydrogenase fold. Native crystal structure

Released:
DOI: 10.2210/pdb5af7/pdb
PDB entry 5af7 coloured by chain, front view.
PDB entry 5af7 coloured by chain, side view.
PDB entry 5af7 coloured by chain, top view.
Source organism: Advenella mimigardefordensis DPN7
Primary publication:
3-Sulfinopropionyl-coenzyme A (3SP-CoA) desulfinase from Advenella mimigardefordensis DPN7(T): crystal structure and function of a desulfinase with an acyl-CoA dehydrogenase fold.
Schürmann M, Meijers R, Schneider TR, Steinbüchel A, Cianci M
OpenAccess logo Acta Crystallogr D Biol Crystallogr 71 1360-72 (2015)
PMID: 26057676

Function and Biology Details

Reaction catalysed: 
3-sulfinopropanoyl-CoA + H(2)O = propanoyl-CoA + sulfite
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125536 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-sulfinopropanoyl-CoA desulfinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 401 amino acids
Theoretical weight: 43.58 KDa
Source organism: Advenella mimigardefordensis DPN7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: K4L7X3 (Residues: 1-401; Coverage: 100%)
Gene names: MIM_c31390, acd
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P21212
Unit cell:
a: 75.674Å b: 100.652Å c: 118.573Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

An integrated pipeline for sample preparation and characterization at the EMBL@PETRA3 synchrotron facilities.
Boivin et al. (2016)
2 more

5 mentions without citation

Reduced Anthocyanins in Petioles codes for a GST anthocyanin transporter that is essential for the foliage and fruit coloration in strawberry.
Luo et al. (2018)
4 more