› 5afa

X-ray diffraction
2.19Å resolution

Crystal structure of Laccase from Thermus thermophilus HB27 complexed with Ag, crystal of the holoenzyme soaked for 30 m in 5 mM AgNO3 at 278 K.

Released:
DOI: 10.2210/pdb5afa/pdb
PDB entry 5afa coloured by chain, front view.
PDB entry 5afa coloured by chain, side view.
PDB entry 5afa coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Entry authors: Jimenez-Arroyo N, Rudino-Pinera E

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180825 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Laccase Chain: A
Molecule details ›
Chain: A
Length: 439 amino acids
Theoretical weight: 48.79 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q72HW2 (Residues: 24-462; Coverage: 95%)
Gene name: TT_C1370
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

5 bound ligands:
Ligand MPD 6 x MPD
Ligand MRD 8 x MRD
Ligand AG 3 x AG
Ligand CU 4 x CU
Ligand OH 1 x OH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: C2221
Unit cell:
a: 93.706Å b: 110.579Å c: 96.547Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.146 0.194
Expression system: Escherichia coli BL21

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