› 5aqb

X-ray diffraction
1.37Å resolution

DARPin-based Crystallization Chaperones exploit Molecular Geometry as a Screening Dimension in Protein Crystallography

Released:
DOI: 10.2210/pdb5aqb/pdb
PDB entry 5aqb coloured by chain, front view.
PDB entry 5aqb coloured by chain, side view.
PDB entry 5aqb coloured by chain, top view.
Source organisms:
Primary publication:
DARPin-Based Crystallization Chaperones Exploit Molecular Geometry as a Screening Dimension in Protein Crystallography.
Batyuk A, Wu Y, Honegger A, Heberling MM, Plückthun A
J Mol Biol 428 1574-88 (2016)
PMID: 26975886

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154541 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
3G61_DB15V4 Chain: A
Molecule details ›
Chain: A
Length: 428 amino acids
Theoretical weight: 46.25 KDa
Source organism: synthetic construct
Expression system: Escherichia coli K-12
Structure domains:
Green fluorescent protein Chain: B
Molecule details ›
Chain: B
Length: 229 amino acids
Theoretical weight: 26.06 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P42212 (Residues: 2-231; Coverage: 97%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 90.15Å b: 96.17Å c: 92.78Å
α: 90° β: 118.99° γ: 90°
R-values:
R R work R free
0.157 0.156 0.177
Expression system: Escherichia coli K-12

Quick links

Citations

1 review citation

The "Sticky Patch" Model of Crystallization and Modification of Proteins for Enhanced Crystallizability.
Derewenda et al. (2017)
11 other articles

1 mention without citation

Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity.
Hansen et al. (2017)