› 5cft

X-ray diffraction
1.5Å resolution

Crystal Structure of ANT(2")-Ia in complex with AMPCPP and gentamicin C1

Released:
DOI: 10.2210/pdb5cft/pdb
PDB entry 5cft coloured by chain, front view.
PDB entry 5cft coloured by chain, side view.
PDB entry 5cft coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural Analysis of the Tobramycin and Gentamicin Clinical Resistome Reveals Limitations for Next-generation Aminoglycoside Design.
Bassenden AV, Rodionov D, Shi K, Berghuis AM
ACS Chem Biol 11 1339-46 (2016)
PMID: 26900880

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180517 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AadB Chain: A
Molecule details ›
Chain: A
Length: 185 amino acids
Theoretical weight: 20.96 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6X3H6 (Residues: 73-249; Coverage: 71%)
Gene names: TNCP6, aadB
Sequence domains: Aminoglycoside-2''-adenylyltransferase
Structure domains: Beta Polymerase; domain 2

Ligands and Environments

4 bound ligands:
Ligand MN 2 x MN
Ligand APC 1 x APC
Ligand 51G 1 x 51G
Ligand P6G 1 x P6G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 40.58Å b: 46.03Å c: 89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.147 0.174
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Antibiotic Combination Therapy: A Strategy to Overcome Bacterial Resistance to Aminoglycoside Antibiotics.
Wang et al. (2022)
3 more

2 mentions without citation

Overcoming Aminoglycoside Enzymatic Resistance: Design of Novel Antibiotics and Inhibitors.
Zárate et al. (2018)
1 more