› 5dez

X-ray diffraction
2.3Å resolution

Crystal structure of AcMNPV Chitinase A

Released:
DOI: 10.2210/pdb5dez/pdb
PDB entry 5dez coloured by chain, front view.
PDB entry 5dez coloured by chain, side view.
PDB entry 5dez coloured by chain, top view.
Source organism: Autographa californica nucleopolyhedrovirus
Entry authors: Mou T-C, Sprang SR

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180711 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
GH18 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 551 amino acids
Theoretical weight: 61.59 KDa
Source organism: Autographa californica nucleopolyhedrovirus
Expression system: Alphabaculovirus
UniProt:
  • Canonical: Q70SQ1 (Residues: 1-551; Coverage: 100%)
Gene names: ACNVgp127, Ac-ChiA, ChiA, chitinase
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand NAG 7 x NAG
Ligand CL 3 x CL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 96.931Å b: 111.927Å c: 129.516Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.229
Expression system: Alphabaculovirus

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