› 5dph

X-ray diffraction
1.42Å resolution

sfGFP mutant - 149 p-cyano-L-phenylalanine

Released:
DOI: 10.2210/pdb5dph/pdb
PDB entry 5dph coloured by chain, front view.
PDB entry 5dph coloured by chain, side view.
PDB entry 5dph coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Probing the effectiveness of spectroscopic reporter unnatural amino acids: a structural study.
Dippel AB, Olenginski GM, Maurici N, Liskov MT, Brewer SH, Phillips-Piro CM
Acta Crystallogr D Struct Biol 72 121-30 (2016)
PMID: 26894540

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 26.96 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand CO2 2 x CO2
Ligand MG 7 x MG
2 modified residues:
Ligand CRO 2 x CRO
Ligand 4CF 2 x 4CF

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P61
Unit cell:
a: 47.27Å b: 47.27Å c: 344.55Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.18 0.208
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Measuring Electric Fields in Biological Matter Using the Vibrational Stark Effect of Nitrile Probes.
Slocum et al. (2018)
7 other articles

3 mentions without citation

Probing the effectiveness of spectroscopic reporter unnatural amino acids: a structural study.
Dippel et al. (2016)
2 more