› 5e7o

X-ray diffraction
2.4Å resolution

Crystal structure of the perchlorate reductase PcrAB mutant W461E of PcrA from Azospira suillum PS

Released:
DOI: 10.2210/pdb5e7o/pdb
PDB entry 5e7o coloured by chain, front view.
PDB entry 5e7o coloured by chain, side view.
PDB entry 5e7o coloured by chain, top view.
Source organism: Azospira oryzae PS
Primary publication:
Perchlorate Reductase Is Distinguished by Active Site Aromatic Gate Residues.
Youngblut MD, Tsai CL, Clark IC, Carlson HK, Maglaqui AP, Gau-Pan PS, Redford SA, Wong A, Tainer JA, Coates JD
J Biol Chem 291 9190-202 (2016)
PMID: 26940877

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124632 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
4Fe-4S Mo/W bis-MGD-type domain-containing protein Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 899 amino acids
Theoretical weight: 102.01 KDa
Source organism: Azospira oryzae PS
Expression system: Azospira oryzae PS
UniProt:
  • Canonical: G8QM55 (Residues: 29-927; Coverage: 100%)
Gene name: Dsui_0149
Sequence domains:
4Fe-4S ferredoxin-type domain-containing protein Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 333 amino acids
Theoretical weight: 37.07 KDa
Source organism: Azospira oryzae PS
Expression system: Azospira oryzae PS
UniProt:
  • Canonical: G8QM54 (Residues: 1-333; Coverage: 100%)
Gene name: Dsui_0148
Sequence domains: 4Fe-4S dicluster domain

Ligands and Environments

7 bound ligands:
Ligand SF4 24 x SF4
Ligand MO 6 x MO
Ligand MGD 6 x MGD
Ligand MD1 6 x MD1
Ligand EDO 29 x EDO
Ligand GOL 2 x GOL
Ligand F3S 6 x F3S
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P21
Unit cell:
a: 135.588Å b: 253.134Å c: 135.797Å
α: 90° β: 119.77° γ: 90°
R-values:
R R work R free
0.198 0.195 0.249
Expression system: Azospira oryzae PS

Quick links

Citations

6 review citations

(Per)chlorate in Biology on Earth and Beyond.
Youngblut et al. (2016)
5 more

1 mention without citation

Perchlorate Reductase Is Distinguished by Active Site Aromatic Gate Residues.
Youngblut et al. (2016)