› 5env

X-ray diffraction
3Å resolution

YEAST ALCOHOL DEHYDROGENASE WITH BOUND COENZYME

Released:
DOI: 10.2210/pdb5env/pdb
PDB entry 5env coloured by chain, front view.
PDB entry 5env coloured by chain, side view.
PDB entry 5env coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Mechanistic implications from structures of yeast alcohol dehydrogenase complexed with coenzyme and an alcohol.
Plapp BV, Charlier HA, Ramaswamy S
Arch Biochem Biophys 591 35-42 (2016)
PMID: 26743849

Function and Biology Details

Reactions catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Allyl alcohol + NADP(+) = acrolein + NADPH
(R)-lactaldehyde + NAD(+) = 2-oxopropanal + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132407 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 36.76 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P00330 (Residues: 2-348; Coverage: 100%)
Gene names: ADC1, ADH1, O0947, YOL086C
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
2 bound ligands:
Ligand ZN 4 x ZN
Ligand ETF 2 x ETF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P321
Unit cell:
a: 146.256Å b: 146.256Å c: 65.98Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.135 0.132 0.197
Expression system: Saccharomyces cerevisiae S288C

Quick links

Citations

2 review citations

Enzyme activation through the utilization of intrinsic dianion binding energy.
Amyes et al. (2017)
1 more

10 mentions without citation

Effects of Ionic Liquids on Metalloproteins.
Patel et al. (2021)
9 more