› 5ffl

X-ray diffraction
1.6Å resolution

Crystal structure of mouse CD300lf at 1.6 Angstroms resolution.

Released:
DOI: 10.2210/pdb5ffl/pdb
PDB entry 5ffl coloured by chain, front view.
PDB entry 5ffl coloured by chain, side view.
PDB entry 5ffl coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Discovery of a proteinaceous cellular receptor for a norovirus.
Orchard RC, Wilen CB, Doench JG, Baldridge MT, McCune BT, Lee YC, Lee S, Pruett-Miller SM, Nelson CA, Fremont DH, Virgin HW
Science 353 933-6 (2016)
PMID: 27540007

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180325 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CMRF35-like molecule 1 Chain: A
Molecule details ›
Chain: A
Length: 113 amino acids
Theoretical weight: 12.79 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6SJQ7 (Residues: 20-128; Coverage: 34%)
  • Best match: Q6SJQ7-2 (Residues: 20-131)
Gene names: Cd300lf, Clm1, Lmir3
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand EPE 1 x EPE
Ligand NA 1 x NA
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 27.633Å b: 53.347Å c: 70.516Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.16 0.196
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

53 review citations

Am I ready for CRISPR? A user's guide to genetic screens.
Doench JG. (2018)
52 more

3 mentions without citation

Discovery of a proteinaceous cellular receptor for a norovirus.
Orchard et al. (2016)
2 more