5g15

X-ray diffraction
2.06Å resolution

Structure Aurora A (122-403) bound to activating monobody Mb1 and AMPPCP

Released:
Primary publication:
Allosteric modulation of a human protein kinase with monobodies.
Proc Natl Acad Sci U S A 116 13937-13942 (2019)
PMID: 31239342

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127234 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aurora kinase A Chain: A
Molecule details ›
Chain: A
Length: 282 amino acids
Theoretical weight: 32.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14965 (Residues: 122-403; Coverage: 70%)
Gene names: AIK, AIRK1, ARK1, AURA, AURKA, AYK1, BTAK, IAK1, STK15, STK6
Sequence domains: Protein kinase domain
Structure domains:
MB1 MONOBODY Chain: B
Molecule details ›
Chain: B
Length: 96 amino acids
Theoretical weight: 10.58 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: I222
Unit cell:
a: 75.339Å b: 91.361Å c: 143.715Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.274
Expression system: Escherichia coli BL21(DE3)