› 5gpr

X-ray diffraction
3.23Å resolution

Crystal structure of chitinase-h from Ostrinia furnacalis

Released:
DOI: 10.2210/pdb5gpr/pdb
PDB entry 5gpr coloured by chain, front view.
PDB entry 5gpr coloured by chain, side view.
PDB entry 5gpr coloured by chain, top view.
Source organism: Ostrinia furnacalis
Primary publication:
Structure, Catalysis, and Inhibition of OfChi-h, the Lepidoptera-exclusive Insect Chitinase.
Liu T, Chen L, Zhou Y, Jiang X, Duan Y, Yang Q
J Biol Chem 292 2080-2088 (2017)
PMID: 28053084

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175444 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GH18 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 553 amino acids
Theoretical weight: 61.07 KDa
Source organism: Ostrinia furnacalis
Expression system: Komagataella phaffii CBS 7435
UniProt:
  • Canonical: Q4AE59 (Residues: 1-553; Coverage: 100%)
Gene name: OfChi-h
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Unit cell:
a: 48.908Å b: 114.419Å c: 122.639Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.212 0.256
Expression system: Komagataella phaffii CBS 7435

Quick links

Citations

4 review citations

Chitinase: diversity, limitations, and trends in engineering for suitable applications.
Oyeleye et al. (2018)
3 more

2 mentions without citation

Structure, Catalysis, and Inhibition of OfChi-h, the Lepidoptera-exclusive Insect Chitinase.
Liu et al. (2017)
1 more