› 5gx2

X-ray diffraction
1.6Å resolution

Luciferin-regenerating enzyme collected with serial synchrotron rotational crystallography with accumulated dose of 3.4 MGy (3rd measurement)

Released:
DOI: 10.2210/pdb5gx2/pdb
PDB entry 5gx2 coloured by chain, front view.
PDB entry 5gx2 coloured by chain, side view.
PDB entry 5gx2 coloured by chain, top view.
Source organism: Photinus pyralis
Primary publication:
Development of a dose-limiting data collection strategy for serial synchrotron rotation crystallography.
Hasegawa K, Yamashita K, Murai T, Nuemket N, Hirata K, Ueno G, Ago H, Nakatsu T, Kumasaka T, Yamamoto M
OpenAccess logo J Synchrotron Radiat 24 29-41 (2017)
PMID: 28009544

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188283 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SMP-30/Gluconolactonase/LRE-like region domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 34.2 KDa
Source organism: Photinus pyralis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q95YI4 (Residues: 1-308; Coverage: 100%)
Sequence domains: SMP-30/Gluconolactonase/LRE-like region
Structure domains: TolB, C-terminal domain

Ligands and Environments

4 bound ligands:
Ligand HG 2 x HG
Ligand MG 1 x MG
Ligand MPD 2 x MPD
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 47.441Å b: 76.781Å c: 84.263Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.158 0.172
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

3D Electron Diffraction for Chemical Analysis: Instrumentation Developments and Innovative Applications.
Gruene et al. (2021)
17 other articles

1 mention without citation

Development of a dose-limiting data collection strategy for serial synchrotron rotation crystallography.
Hasegawa et al. (2017)