› 5gzt

X-ray diffraction
2.1Å resolution

Crystal Structure of Chitinase ChiW from Paenibacillus sp. str. FPU-7 Reveals a Novel Type of Bacterial Cell-Surface-Expressed Multi-Modular Enzyme Machinery

Released:
DOI: 10.2210/pdb5gzt/pdb
PDB entry 5gzt coloured by chain, front view.
PDB entry 5gzt coloured by chain, side view.
PDB entry 5gzt coloured by chain, top view.
Source organism: Paenibacillus sp. FPU-7
Primary publication:
Crystal Structure of Chitinase ChiW from Paenibacillus sp. str. FPU-7 Reveals a Novel Type of Bacterial Cell-Surface-Expressed Multi-Modular Enzyme Machinery.
Itoh T, Hibi T, Suzuki F, Sugimoto I, Fujiwara A, Inaka K, Tanaka H, Ohta K, Fujii Y, Taketo A, Kimoto H
OpenAccess logo PLoS One 11 e0167310 (2016)
PMID: 27907169

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125635 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chitinase Chain: A
Molecule details ›
Chain: A
Length: 108 amino acids
Theoretical weight: 11.37 KDa
Source organism: Paenibacillus sp. FPU-7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: K7ZLW6 (Residues: 198-282; Coverage: 6%)
Gene name: chiW
Chitinase Chain: B
Molecule details ›
Chain: B
Length: 1136 amino acids
Theoretical weight: 123.45 KDa
Source organism: Paenibacillus sp. FPU-7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: K7ZLW6 (Residues: 283-1418; Coverage: 82%)
Gene name: chiW
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand NA 1 x NA
Ligand PO4 1 x PO4
Ligand FMT 10 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P212121
Unit cell:
a: 114.339Å b: 123.486Å c: 130.856Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Structural insights into the mechanisms and specificities of IgG-active endoglycosidases.
Du et al. (2020)
10 other articles

1 mention without citation

The crystal structure of the chitinase ChiA74 of Bacillus thuringiensis has a multidomain assembly.
Juárez-Hernández et al. (2019)