› 5hwp

X-ray diffraction
2.2Å resolution

MvaS with acetylated Cys115 in complex with coenzyme A

Released:
DOI: 10.2210/pdb5hwp/pdb
PDB entry 5hwp coloured by chain, front view.
PDB entry 5hwp coloured by chain, side view.
PDB entry 5hwp coloured by chain, top view.
Source organism: Myxococcus xanthus DK 1622
Primary publication:
Crystal Structure of the HMG-CoA Synthase MvaS from the Gram-Negative Bacterium Myxococcus xanthus.
Bock T, Kasten J, Müller R, Blankenfeldt W
Chembiochem 17 1257-62 (2016)
PMID: 27124816

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + H(2)O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172798 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydroxymethylglutaryl-CoA synthase Chain: A
Molecule details ›
Chain: A
Length: 419 amino acids
Theoretical weight: 44.56 KDa
Source organism: Myxococcus xanthus DK 1622
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1D4I1 (Residues: 1-417; Coverage: 100%)
Gene names: MXAN_4267, mvaS
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments


Cofactor: Ligand COA 1 x COA
2 bound ligands:
Ligand SO4 1 x SO4
Ligand GOL 1 x GOL
1 modified residue:
Ligand SCY 1 x SCY

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P6122
Unit cell:
a: 73.32Å b: 73.32Å c: 277.379Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.176 0.22
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The Syntrophy hypothesis for the origin of eukaryotes revisited.
López-García et al. (2020)
4 other articles