› 5ig2

X-ray diffraction
1.8Å resolution

Crystal structure of a short chain dehydrogenase/reductase SDR from Burkholderia phymatum in complex with NAD

Released:
DOI: 10.2210/pdb5ig2/pdb
PDB entry 5ig2 coloured by chain, front view.
PDB entry 5ig2 coloured by chain, side view.
PDB entry 5ig2 coloured by chain, top view.
Source organism: Paraburkholderia phymatum STM815
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo hexamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109657 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Short-chain dehydrogenase/reductase SDR Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 297 amino acids
Theoretical weight: 31.6 KDa
Source organism: Paraburkholderia phymatum STM815
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B2JGP2 (Residues: 1-289; Coverage: 100%)
Gene name: Bphy_1038
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand NAD 3 x NAD
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2221
Unit cell:
a: 83.52Å b: 187.79Å c: 108.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.142 0.141 0.177
Expression system: Escherichia coli BL21(DE3)

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