› 5ij6

X-ray diffraction
2Å resolution

Crystal structure of Enterococcus faecalis lipoate-protein ligase A (lplA-1) in complex with lipoic acid

Released:
DOI: 10.2210/pdb5ij6/pdb
PDB entry 5ij6 coloured by chain, front view.
PDB entry 5ij6 coloured by chain, side view.
PDB entry 5ij6 coloured by chain, top view.
Source organism: Enterococcus faecalis V583
Entry authors: Hughes SJ, Lyle AG, Song JH, Antoshchenko T, Park H

Function and Biology Details

Reaction catalysed: 
(1a) ATP + (R)-lipoate = lipoyl-AMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182868 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
lipoate--protein ligase Chain: A
Molecule details ›
Chain: A
Length: 355 amino acids
Theoretical weight: 40.78 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q838C1 (Residues: 1-337; Coverage: 100%)
Gene names: EF_0650, lplA-1
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand LPA 1 x LPA
Ligand SO4 1 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 53.011Å b: 70.912Å c: 106.466Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.178 0.207
Expression system: Escherichia coli BL21(DE3)

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