› 5iuw

X-ray diffraction
2.09Å resolution

Crystal Structure of Indole-3-acetaldehyde Dehydrogenase in complexed with NAD+ and IAA

Released:
DOI: 10.2210/pdb5iuw/pdb
PDB entry 5iuw coloured by chain, front view.
PDB entry 5iuw coloured by chain, side view.
PDB entry 5iuw coloured by chain, top view.
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Primary publication:
Indole-3-acetaldehyde dehydrogenase-dependent auxin synthesis contributes to virulence of Pseudomonas syringae strain DC3000.
McClerklin SA, Lee SG, Harper CP, Nwumeh R, Jez JM, Kunkel BN
OpenAccess logo PLoS Pathog 14 e1006811 (2018)
PMID: 29293681

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde dehydrogenase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 497 amino acids
Theoretical weight: 52.78 KDa
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q88BC5 (Residues: 1-497; Coverage: 100%)
Gene name: PSPTO_0092
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NAD 2 x NAD
Ligand IAC 2 x IAC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Unit cell:
a: 80.799Å b: 84.883Å c: 166.938Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.15 0.189
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Metabolomics as an Emerging Tool for the Study of Plant-Pathogen Interactions.
Castro-Moretti et al. (2020)
10 more

5 mentions without citation

Molecular Mechanisms of Bacterial Bioluminescence.
Brodl et al. (2018)
4 more