› 5k7l

Electron Microscopy
3.78Å resolution

Single particle cryo-EM structure of the voltage-gated K+ channel Eag1 bound to the channel inhibitor calmodulin

Released:
DOI: 10.2210/pdb5k7l/pdb
PDB entry 5k7l coloured by chain, front view.
PDB entry 5k7l coloured by chain, side view.
PDB entry 5k7l coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the voltage-gated K⁺ channel Eag1 reveals an alternative voltage sensing mechanism.
Whicher JR, MacKinnon R
Science 353 664-9 (2016)
PMID: 27516594
Related structures: EMD-8215

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143998 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Voltage-gated delayed rectifier potassium channel KCNH1 Chain: A
Molecule details ›
Chain: A
Length: 857 amino acids
Theoretical weight: 97.36 KDa
Source organism: Rattus norvegicus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q63472 (Residues: 1-962; Coverage: 88%)
Gene names: EAG1, Eag, Kcnh1
Sequence domains:
Calmodulin-1 Chain: B
Molecule details ›
Chain: B
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair

Ligands and Environments

2 bound ligands:
Ligand NAG 1 x NAG
Ligand Y01 1 x Y01
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.78Å
Relevant EMDB volumes: EMD-8215
Expression system: Homo sapiens

Quick links

Citations

46 review citations

Cryo-EM in drug discovery: achievements, limitations and prospects.
Renaud et al. (2018)
45 more

67 mentions without citation

Structural insights into the mechanisms of CNBD channel function.
James et al. (2018)
66 more