› 5m8h

X-ray diffraction
2.34Å resolution

ATP phosphoribosyltransferase (HisZG ATPPRT) from Psychrobacter arcticus

Released:
DOI: 10.2210/pdb5m8h/pdb
PDB entry 5m8h coloured by chain, front view.
PDB entry 5m8h coloured by chain, side view.
PDB entry 5m8h coloured by chain, top view.
Source organism: Psychrobacter arcticus 273-4
Primary publication:
Kinetics and Structure of a Cold-Adapted Hetero-Octameric ATP Phosphoribosyltransferase.
Stroek R, Ge Y, Talbot PD, Glok MK, Bernaś KE, Thomson CM, Gould ER, Alphey MS, Liu H, Florence GJ, Naismith JH, da Silva RG
Biochemistry 56 793-803 (2017)
PMID: 28092443

Function and Biology Details

Reaction catalysed: 
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175515 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATP phosphoribosyltransferase regulatory subunit Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 388 amino acids
Theoretical weight: 43.13 KDa
Source organism: Psychrobacter arcticus 273-4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4FTX3 (Residues: 1-387; Coverage: 100%)
Gene names: Psyc_0676, hisZ
Sequence domains: Histidyl-tRNA synthetase
ATP phosphoribosyltransferase Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 232 amino acids
Theoretical weight: 25.24 KDa
Source organism: Psychrobacter arcticus 273-4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4FQF7 (Residues: 1-231; Coverage: 100%)
Gene names: Psyc_1903, hisG
Sequence domains: ATP phosphoribosyltransferase
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

3 bound ligands:
Ligand SR 10 x SR
Ligand CL 15 x CL
Ligand MPD 12 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 94.02Å b: 146.73Å c: 101.9Å
α: 90° β: 102.4° γ: 90°
R-values:
R R work R free
0.23 0.227 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Advances in cold-adapted enzymes derived from microorganisms.
Liu et al. (2023)
6 other articles

2 mentions without citation

Allosteric rescue of catalytically impaired ATP phosphoribosyltransferase variants links protein dynamics to active-site electrostatic preorganisation.
Fisher et al. (2022)
1 more