› 5ma3

X-ray diffraction
1.7Å resolution

GFP-binding DARPin fusion gc_R11

Released:
DOI: 10.2210/pdb5ma3/pdb
PDB entry 5ma3 coloured by chain, front view.
PDB entry 5ma3 coloured by chain, side view.
PDB entry 5ma3 coloured by chain, top view.
Source organisms:
Primary publication:
Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity.
Hansen S, Stüber JC, Ernst P, Koch A, Bojar D, Batyuk A, Plückthun A
OpenAccess logo Sci Rep 7 16292 (2017)
PMID: 29176615

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154542 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Green fluorescent protein Chain: B
Molecule details ›
Chain: B
Length: 243 amino acids
Theoretical weight: 27.45 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein
R11 Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 31.65 KDa
Source organism: synthetic construct
Expression system: Escherichia coli K-12

Ligands and Environments

1 bound ligand:
Ligand EDO 6 x EDO
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 59.85Å b: 90.61Å c: 60.92Å
α: 90° β: 103.42° γ: 90°
R-values:
R R work R free
0.163 0.161 0.192
Expression system: Escherichia coli K-12

Quick links

Citations

1 review citation

Advances in targeted degradation of endogenous proteins.
Röth et al. (2019)
24 other articles

2 mentions without citation

Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity.
Hansen et al. (2017)
1 more