› 5mh5

X-ray diffraction
1.4Å resolution

D-2-hydroxyacid dehydrogenases (D2-HDH) from Haloferax mediterranei in complex with 2-keto-hexanoic acid and NADP+ (1.4 A resolution)

Released:
DOI: 10.2210/pdb5mh5/pdb
PDB entry 5mh5 coloured by chain, front view.
PDB entry 5mh5 coloured by chain, side view.
PDB entry 5mh5 coloured by chain, top view.
Source organism: Haloferax mediterranei ATCC 33500
Entry authors: Bisson C, Baker PJ, Domenech Perez J, Pramanpol N, Harding SE, Rice DW, Ferrer J

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174099 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-2-hydroxyacid dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 308 amino acids
Theoretical weight: 33.37 KDa
Source organism: Haloferax mediterranei ATCC 33500
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2VEQ7 (Residues: 1-308; Coverage: 100%)
Gene names: HFX_2024, ddh, serA5
Sequence domains: D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

4 bound ligands:
Ligand MG 21 x MG
Ligand 7N5 2 x 7N5
Ligand NAP 2 x NAP
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 62.53Å b: 87.4Å c: 66.1Å
α: 90° β: 96.34° γ: 90°
R-values:
R R work R free
0.137 0.136 0.167
Expression system: Escherichia coli BL21(DE3)

Quick links