› 5mh6

X-ray diffraction
1.35Å resolution

D-2-hydroxyacid dehydrogenases (D2-HDH) from Haloferax mediterranei in complex with 2-ketohexanoic acid and NAD+ (1.35 A resolution)

Released:
DOI: 10.2210/pdb5mh6/pdb
PDB entry 5mh6 coloured by chain, front view.
PDB entry 5mh6 coloured by chain, side view.
PDB entry 5mh6 coloured by chain, top view.
Source organism: Haloferax mediterranei ATCC 33500
Entry authors: Bisson C, Baker PJ, Domenech Perez J, Pramanpol N, Harding SE, Rice DW, Ferrer J

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174099 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-2-hydroxyacid dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 308 amino acids
Theoretical weight: 33.37 KDa
Source organism: Haloferax mediterranei ATCC 33500
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2VEQ7 (Residues: 1-308; Coverage: 100%)
Gene names: HFX_2024, ddh, serA5
Sequence domains: D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain

Ligands and Environments

6 bound ligands:
Ligand EDO 22 x EDO
Ligand SO4 4 x SO4
Ligand MG 24 x MG
Ligand NAD 4 x NAD
Ligand 7N5 4 x 7N5
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P1
Unit cell:
a: 66.455Å b: 75.008Å c: 77.555Å
α: 109.11° β: 107.55° γ: 95.91°
R-values:
R R work R free
0.128 0.126 0.162
Expression system: Escherichia coli BL21(DE3)

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