› 5n6x

X-ray diffraction
1.75Å resolution

Crystal structure of the Legionella effector WipA

Released:
DOI: 10.2210/pdb5n6x/pdb
PDB entry 5n6x coloured by chain, front view.
PDB entry 5n6x coloured by chain, side view.
PDB entry 5n6x coloured by chain, top view.
Source organism: Legionella pneumophila
Primary publication:
Structure of the WipA protein reveals a novel tyrosine protein phosphatase effector from Legionella pneumophila.
Pinotsis N, Waksman G
OpenAccess logo J Biol Chem 292 9240-9251 (2017)
PMID: 28389563

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177143 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dot/Icm T4SS effector WipA Chains: A, B
Molecule details ›
Chains: A, B
Length: 414 amino acids
Theoretical weight: 48.17 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5GA16 (Residues: 24-435; Coverage: 79%)
Gene names: JBJ86_01095, wipA
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand MN 4 x MN
Ligand PO4 2 x PO4
Ligand BR 6 x BR
Ligand MPD 2 x MPD
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P41212
Unit cell:
a: 80.65Å b: 80.65Å c: 321.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.215
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Legionella pneumophila-mediated host posttranslational modifications.
Yang et al. (2023)
4 other articles

2 mentions without citation

Structure of the WipA protein reveals a novel tyrosine protein phosphatase effector from Legionella pneumophila.
Pinotsis et al. (2017)
1 more