5ocs

X-ray diffraction
1.7Å resolution

Ene-reductase (ER/OYE) from Ralstonia (Cupriavidus) metallidurans

Released:

Function and Biology Details

Reaction catalysed:
NADPH + acceptor = NADP(+) + reduced acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-172950 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 371 amino acids
Theoretical weight: 40.19 KDa
Source organism: Cupriavidus metallidurans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q1LDQ5 (Residues: 1-371; Coverage: 100%)
Gene name: Rmet_4859
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 77.97Å b: 95.04Å c: 208.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.197
Expression system: Escherichia coli BL21(DE3)