› 5ogt

X-ray diffraction
2.3Å resolution

Thermus scotoductus SA-01 Ene-reductase triple mutant TsER_C25D_I67T_A102H

Released:
DOI: 10.2210/pdb5ogt/pdb
PDB entry 5ogt coloured by chain, front view.
PDB entry 5ogt coloured by chain, side view.
PDB entry 5ogt coloured by chain, top view.
Source organism: Thermus scotoductus SA-01
Entry authors: Opperman DJ, Hoebenreich S, Nett N

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123113 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 349 amino acids
Theoretical weight: 38.09 KDa
Source organism: Thermus scotoductus SA-01
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E8PRF1 (Residues: 1-347; Coverage: 100%)
Gene name: TSC_p800090
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand FMN 4 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P2
Unit cell:
a: 100.27Å b: 103.24Å c: 102.72Å
α: 90° β: 113.28° γ: 90°
R-values:
R R work R free
0.206 0.204 0.244
Expression system: Escherichia coli BL21(DE3)

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