› 5t5q

X-ray diffraction
1.95Å resolution

Crystal structure of Short-chain dehydrogenase/reductase SDR:Glucose/ribitol dehydrogenase from Brucella melitensis

Released:
DOI: 10.2210/pdb5t5q/pdb
PDB entry 5t5q coloured by chain, front view.
PDB entry 5t5q coloured by chain, side view.
PDB entry 5t5q coloured by chain, top view.
Source organism: Brucella abortus 2308
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174162 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Short-chain dehydrogenase/reductase SDR:Glucose/ribitol dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 247 amino acids
Theoretical weight: 26.23 KDa
Source organism: Brucella abortus 2308
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2YL80 (Residues: 1-245; Coverage: 100%)
Gene name: BAB2_0029
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
2 bound ligands:
Ligand SO4 2 x SO4
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 64.85Å b: 108.15Å c: 65.66Å
α: 90° β: 103.12° γ: 90°
R-values:
R R work R free
0.176 0.174 0.219
Expression system: Escherichia coli BL21(DE3)

Quick links