› 5u0m

X-ray diffraction
3.08Å resolution

Fatty aldehyde dehydrogenase from Marinobacter aquaeolei VT8 and cofactor complex

Released:
DOI: 10.2210/pdb5u0m/pdb
PDB entry 5u0m coloured by chain, front view.
PDB entry 5u0m coloured by chain, side view.
PDB entry 5u0m coloured by chain, top view.
Source organism: Marinobacter nauticus VT8
Primary publication:
Five Fatty Aldehyde Dehydrogenase Enzymes from Marinobacter and Acinetobacter spp. and Structural Insights into the Aldehyde Binding Pocket.
Bertram JH, Mulliner KM, Shi K, Plunkett MH, Nixon P, Serratore NA, Douglas CJ, Aihara H, Barney BM
Appl Environ Microbiol 83 (2017)
PMID: 28389542

Function and Biology Details

Reaction catalysed: 
N-succinyl-L-glutamate 5-semialdehyde + NAD(+) + H(2)O = N-succinyl-L-glutamate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106724 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-succinylglutamate 5-semialdehyde dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 497 amino acids
Theoretical weight: 53.37 KDa
Source organism: Marinobacter nauticus VT8
Expression system: Escherichia coli
UniProt:
  • Canonical: A1U5W8 (Residues: 2-491; Coverage: 100%)
Gene names: Maqu_3316, astD
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
2 bound ligands:
Ligand EDO 3 x EDO
Ligand CIT 2 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P41212
Unit cell:
a: 99.347Å b: 99.347Å c: 254.425Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.219 0.273
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Microbial synthesis of medium-chain chemicals from renewables.
Sarria et al. (2017)
1 more

1 mention without citation

Five Fatty Aldehyde Dehydrogenase Enzymes from Marinobacter and Acinetobacter spp. and Structural Insights into the Aldehyde Binding Pocket.
Bertram et al. (2017)