› 5utu

X-ray diffraction
2.65Å resolution

2.65 Angstrom Resolution Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with SAH and NAD

Released:
DOI: 10.2210/pdb5utu/pdb
PDB entry 5utu coloured by chain, front view.
PDB entry 5utu coloured by chain, side view.
PDB entry 5utu coloured by chain, top view.
Source organism: Cryptosporidium parvum Iowa II
Entry authors: Minasov G, Shuvalova L, Dubrovska I, Winsor J, Stam J, Kwon K, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176910 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosylhomocysteinase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 498 amino acids
Theoretical weight: 56.2 KDa
Source organism: Cryptosporidium parvum Iowa II
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5CPH1 (Residues: 1-495; Coverage: 100%)
Gene name: cgd3_80
Sequence domains:
Structure domains:

Ligands and Environments

8 bound ligands:
Ligand NAD 8 x NAD
Ligand SAH 8 x SAH
Ligand CL 11 x CL
Ligand SO4 22 x SO4
Ligand P33 3 x P33
Ligand P6G 2 x P6G
Ligand ADN 2 x ADN
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 107.906Å b: 185.834Å c: 121.619Å
α: 90° β: 97.98° γ: 90°
R-values:
R R work R free
0.172 0.17 0.202
Expression system: Escherichia coli BL21(DE3)

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