› 5uyb

X-ray diffraction
1.95Å resolution

YfeA from M9 minimal media supplemented with manganese - Fe wavelength

Released:
DOI: 10.2210/pdb5uyb/pdb
PDB entry 5uyb coloured by chain, front view.
PDB entry 5uyb coloured by chain, side view.
PDB entry 5uyb coloured by chain, top view.
Source organism: Yersinia pestis
Primary publication:
Crystal structure of Yersinia pestis virulence factor YfeA reveals two polyspecific metal-binding sites.
Radka CD, DeLucas LJ, Wilson LS, Lawrenz MB, Perry RD, Aller SG
OpenAccess logo Acta Crystallogr D Struct Biol 73 557-572 (2017)
PMID: 28695856

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176385 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron-binding protein YfeA Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 36.05 KDa
Source organism: Yersinia pestis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56952 (Residues: 1-311; Coverage: 100%)
Gene names: YPO2439, YP_2227, y1897, yfeA
Sequence domains: Zinc-uptake complex component A periplasmic
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

1 bound ligand:
Ligand FE 1 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 41.746Å b: 52.186Å c: 112.744Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.165 0.202
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Iron Acquisition Systems of Gram-negative Bacterial Pathogens Define TonB-Dependent Pathways to Novel Antibiotics.
Klebba et al. (2021)
3 more

1 mention without citation

Crystal structure of Yersinia pestis virulence factor YfeA reveals two polyspecific metal-binding sites.
Radka et al. (2017)