› 5uz7

Electron Microscopy
4.1Å resolution

Volta phase plate cryo-electron microscopy structure of a calcitonin receptor-heterotrimeric Gs protein complex

Released:
DOI: 10.2210/pdb5uz7/pdb
PDB entry 5uz7 coloured by chain, front view.
PDB entry 5uz7 coloured by chain, side view.
PDB entry 5uz7 coloured by chain, top view.
Source organisms:
Primary publication:
Phase-plate cryo-EM structure of a class B GPCR-G-protein complex.
Liang YL, Khoshouei M, Radjainia M, Zhang Y, Glukhova A, Tarrasch J, Thal DM, Furness SGB, Christopoulos G, Coudrat T, Danev R, Baumeister W, Miller LJ, Christopoulos A, Kobilka BK, Wootten D, Skiniotis G, Sexton PM
OpenAccess logo Nature 546 118-123 (2017)
PMID: 28437792
Related structures: EMD-8623

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-542305 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 44.33 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 96%)
  • Best match: P63092-2 (Residues: 1-380)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 351 amino acids
Theoretical weight: 38.74 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 68 amino acids
Theoretical weight: 7.56 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-68; Coverage: 96%)
Gene name: GNG2
Sequence domains: GGL domain
NANOBODY 35 Chain: N
Molecule details ›
Chain: N
Length: 138 amino acids
Theoretical weight: 15.14 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Calcitonin receptor Chain: R
Molecule details ›
Chain: R
Length: 501 amino acids
Theoretical weight: 58.47 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P30988 (Residues: 25-474; Coverage: 100%)
  • Best match: P30988-3 (Residues: 1-427)
Gene name: CALCR
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-8623
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

79 review citations

The Molecular Basis of G Protein-Coupled Receptor Activation.
Weis et al. (2018)
78 more

40 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
39 more