› 5v4v

X-ray diffraction
1.8Å resolution

Saccharomyces cerevisiae Old Yellow Enzyme 3

Released:
DOI: 10.2210/pdb5v4v/pdb
PDB entry 5v4v coloured by chain, front view.
PDB entry 5v4v coloured by chain, side view.
PDB entry 5v4v coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Entry author: Stewart JD

Function and Biology Details

Reaction catalysed: 
NADPH + acceptor = NADP(+) + reduced acceptor
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154460 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH dehydrogenase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 400 amino acids
Theoretical weight: 44.97 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P41816 (Residues: 1-400; Coverage: 100%)
Gene names: OYE3, P2291, YPL171C
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
2 bound ligands:
Ligand NCA 2 x NCA
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 61.214Å b: 107.762Å c: 141.071Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.148 0.189
Expression system: Escherichia coli

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