› 5w25

X-ray diffraction
2.65Å resolution

Crystal structure of Aspartyl-tRNA synthetase from Mycobacterium tuberculosis complexed with L-Aspartic Acid

Released:
DOI: 10.2210/pdb5w25/pdb
PDB entry 5w25 coloured by chain, front view.
PDB entry 5w25 coloured by chain, side view.
PDB entry 5w25 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-161434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate--tRNA(Asp/Asn) ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 616 amino acids
Theoretical weight: 67.44 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WFW3 (Residues: 2-594; Coverage: 100%)
Gene names: MTCY227.29, Rv2572c, aspS
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ASP 2 x ASP
Ligand LYS 3 x LYS
Ligand GLU 2 x GLU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 64.72Å b: 158.24Å c: 75.74Å
α: 90° β: 93.77° γ: 90°
R-values:
R R work R free
0.226 0.225 0.245
Expression system: Escherichia coli BL21(DE3)

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