› 5w6b

X-ray diffraction
1.74Å resolution

Phosphotriesterase variant S1

Released:
DOI: 10.2210/pdb5w6b/pdb
PDB entry 5w6b coloured by chain, front view.
PDB entry 5w6b coloured by chain, side view.
PDB entry 5w6b coloured by chain, top view.
Source organism: Brevundimonas diminuta
Entry authors: Miton CM, Campbell EC, Jackson CJ, Tokuriki N

Function and Biology Details

Reaction catalysed: 
An aryl dialkyl phosphate + H(2)O = dialkyl phosphate + an aryl alcohol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141251 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Parathion hydrolase Chains: A, G
Molecule details ›
Chains: A, G
Length: 333 amino acids
Theoretical weight: 36.01 KDa
Source organism: Brevundimonas diminuta
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A434 (Residues: 34-365; Coverage: 99%)
Gene name: opd
Sequence domains: Phosphotriesterase family
Structure domains: Metal-dependent hydrolases

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand CAC 2 x CAC
Ligand MPD 3 x MPD
1 modified residue:
Ligand KCX 2 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21212
Unit cell:
a: 85.66Å b: 85.79Å c: 88.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.198
Expression system: Escherichia coli BL21(DE3)

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