› 5wup

X-ray diffraction
2.3Å resolution

Crystal structure of a insect group III chitinase (CAD1) from Ostrinia furnacalis

Released:
DOI: 10.2210/pdb5wup/pdb
PDB entry 5wup coloured by chain, front view.
PDB entry 5wup coloured by chain, side view.
PDB entry 5wup coloured by chain, top view.
Source organism: Ostrinia furnacalis
Primary publication:
The deduced role of a chitinase containing two nonsynergistic catalytic domains.
Liu T, Zhu W, Wang J, Zhou Y, Duan Y, Qu M, Yang Q
OpenAccess logo Acta Crystallogr D Struct Biol 74 30-40 (2018)
PMID: 29372897

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197558 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chitinase Chain: A
Molecule details ›
Chain: A
Length: 482 amino acids
Theoretical weight: 54.04 KDa
Source organism: Ostrinia furnacalis
Expression system: Komagataella phaffii CBS 7435
UniProt:
  • Canonical: U5LUV7 (Residues: 1-482; Coverage: 48%)
Sequence domains: Glycosyl hydrolases family 18

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41212
Unit cell:
a: 71.835Å b: 71.835Å c: 193.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.217
Expression system: Komagataella phaffii CBS 7435

Quick links

Citations

2 citation in other articles

A chitinase with two catalytic domains is required for organization of the cuticular extracellular matrix of a beetle.
Noh et al. (2018)
1 more

1 mention without citation

The deduced role of a chitinase containing two nonsynergistic catalytic domains.
Liu et al. (2018)