› 5x33

X-ray diffraction
3.7Å resolution

Leukotriene B4 receptor BLT1 in complex with BIIL260

Released:
DOI: 10.2210/pdb5x33/pdb
PDB entry 5x33 coloured by chain, front view.
PDB entry 5x33 coloured by chain, side view.
PDB entry 5x33 coloured by chain, top view.
Source organisms:
Primary publication:
Na+-mimicking ligands stabilize the inactive state of leukotriene B4 receptor BLT1.
Hori T, Okuno T, Hirata K, Yamashita K, Kawano Y, Yamamoto M, Hato M, Nakamura M, Shimizu T, Yokomizo T, Miyano M, Yokoyama S
Nat Chem Biol 14 262-269 (2018)
PMID: 29309055

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100753 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin; G-protein coupled receptors family 1 profile domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 523 amino acids
Theoretical weight: 57.76 KDa
Source organisms: Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9WTK1 (Residues: 15-213, 214-348; Coverage: 96%)
  • Canonical: A0A097J792 (Residues: 2-161; Coverage: 98%)
Gene names: LTB4R, RB55_p125, e
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand 7Y9 1 x 7Y9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Unit cell:
a: 69.56Å b: 77.62Å c: 135.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.251 0.248 0.296
Expression system: Komagataella pastoris

Quick links

Citations

4 review citations

Structural insights into G-protein-coupled receptor allostery.
Thal et al. (2018)
3 more

21 mentions without citation

Harnessing Ion-Binding Sites for GPCR Pharmacology.
Zarzycka et al. (2019)
20 more