› 5xw1

X-ray diffraction
1.9Å resolution

Crystal Structure of Porcine pancreatic trypsin with tripeptide inhibitor, PRN, at pH10

Released:
DOI: 10.2210/pdb5xw1/pdb
PDB entry 5xw1 coloured by chain, front view.
PDB entry 5xw1 coloured by chain, side view.
PDB entry 5xw1 coloured by chain, top view.
Source organisms:
Entry authors: Saikhedkar NS, Bhoite AS, Giri AP, Kulkarni KA

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133378 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Trypsin Chain: A
Molecule details ›
Chain: A
Length: 133 amino acids
Theoretical weight: 14.24 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P00761 (Residues: 1-133; Coverage: 58%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Trypsin Chain: B
Molecule details ›
Chain: B
Length: 98 amino acids
Theoretical weight: 10.21 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P00761 (Residues: 134-231; Coverage: 42%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Acetylated-Pro-Arg-Asn Inhibitor Chain: C
Molecule details ›
Chain: C
Length: 4 amino acids
Theoretical weight: 412 Da
Source organism: Capsicum annuum
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand MPD 3 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RRCAT INDUS-2 BEAMLINE PX-BL21
Spacegroup: P6122
Unit cell:
a: 83.165Å b: 83.165Å c: 135.232Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.146 0.145 0.173
Expression system: Not provided

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