› 6bv5

X-ray diffraction
1.79Å resolution

Structure of proteinaceous RNase P 1 (PRORP1) from A. thaliana after 45-minute soak with juglone

Released:
DOI: 10.2210/pdb6bv5/pdb
PDB entry 6bv5 coloured by chain, front view.
PDB entry 6bv5 coloured by chain, side view.
PDB entry 6bv5 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Entry authors: Karasik A, Wu N, Fierke CA, Koutmos M

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179276 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteinaceous RNase P 1, chloroplastic/mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 501 amino acids
Theoretical weight: 56.66 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q66GI4 (Residues: 77-572; Coverage: 87%)
Gene names: At2g32230, F22D22.2, PRORP1
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand JUG 1 x JUG
Ligand MN 1 x MN
Ligand ZN 1 x ZN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 41.616Å b: 110.838Å c: 140.743Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.223
Expression system: Escherichia coli BL21(DE3)

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