› 6cmz

X-ray diffraction
2.3Å resolution

2.3 Angstrom Resolution Crystal Structure of Dihydrolipoamide Dehydrogenase from Burkholderia cenocepacia in Complex with FAD and NAD

Released:
DOI: 10.2210/pdb6cmz/pdb
PDB entry 6cmz coloured by chain, front view.
PDB entry 6cmz coloured by chain, side view.
PDB entry 6cmz coloured by chain, top view.
Source organism: Burkholderia cenocepacia J2315
Entry authors: Minasov G, Shuvalova L, Dubrovska I, Kiryukhina O, Grimshaw S, Kwon K, Anderson WF, Satchell KJF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110029 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrolipoyl dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 466 amino acids
Theoretical weight: 48.96 KDa
Source organism: Burkholderia cenocepacia J2315
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B4EEF2 (Residues: 1-463; Coverage: 100%)
Gene names: BCAL1215, lpdV
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 5 x FAD

Cofactor: Ligand NAD 1 x NAD

Cofactor: Ligand FMN 1 x FMN
3 bound ligands:
Ligand CL 2 x CL
Ligand ADP 3 x ADP
Ligand MLT 1 x MLT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 83.565Å b: 107.246Å c: 105.427Å
α: 90° β: 106.09° γ: 90°
R-values:
R R work R free
0.201 0.199 0.253
Expression system: Escherichia coli BL21(DE3)

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